Cryo-Electron Microscopy and Structural Biology
Research Specialist, WUCCI
As a Cyro-EM specialist in the WUCCI, Michael’s primary responsibility is imaging with the Titan Krios G3 Cryo-EM which, includes the preparation of Cryo samples and processing micrograph movies to produce atomic level structures.
Michael is a biochemist by undergraduate training. He has spent 7 years as a Lab Manager at Washington University in St. Louis’ Department of Biochemistry and Molecular Biophysics under Dr. Kathleen Hall studying RNA structure and dynamics. Michael used multi-dimensional NMR and unique fluorescence techniques to study the structure and dynamics of RNA and RNA protein interactions. He has collaborated with Dr. Thomas Cheatham III to help refine RNA molecular dynamics simulation force fields with experimental data..
- B.A. in Chemistry with a minor in Biology, University of Central Missouri, MO, USA
- M.J. Rau, R. Welty, W.T. Stump, & K.B. Hall. (2015). Formation of Tertiary Interactions during rRNA GTPase Center Folding. Journal of Molecular Biology, 427(17), 2799–2815. doi:10.1016/j.jmb.2015.07.013
- M.J. Rau, & K.B. Hall. (2015). 2-aminopurine fluorescence as a probe of local RNA structure and dynamics and global folding. Methods in Enzymology (1st ed., Vol. 558, pp. 99–124). Elsevier Inc. doi:10.1016/bs.mie.2015.01.006
- M.J. Rau, W.T. Stump, & K.B. Hall. (2012). Intrinsic flexibility of snRNA hairpin loops facilitates protein binding. RNA (New York, N.Y.), 18, 1984–95. doi:10.1261/rna.035006.112