WUCCI Rate Schedule as of March 1, 2023

 Washington UniversityWashington UniversityExternal AcademicCommercial 1
 Peak 2Off-peakSingle-rateSingle-rate
LIGHT MICROSCOPY
Fluorescence / Slide Scanning
(User)
$30$22$45$90
Fluorescence / Slide Scanning
(Assisted)
$44$65$131
Fluorescence / Slide Scanning
(Daily)
$220$330$660
Confocal LSM
(User)
$40$31$62$125
Confocal LSM
(Assisted)
$58$90$181
Confocal LSM
(Daily)
$310$465$930
Two-Photon LSM
(User)
$40$31$62$125
Two-Photon LSM
(Assisted)
$58$90$181
Two-Photon LSM
(Daily)
$310$465$930
Lightsheet 7
(User)
$33$25$50$100
Lightsheet 7
(Cleared samples- Assisted)
$36$54$108
Lightsheet 7
(Aqueous samples- Assisted)
$48$72$144
Lightsheet 7
(Daily)3
$250$375$750
Lattice Lightsheet
(User)
$33$25$50$100
Lattice Lightsheet
(Assisted)
$36$54$108
Lattice Lightsheet
(Daily)
$250$375$750
Live-Cell Confocal
(User)
$39$30$60$120
Live-Cell Confocal
(Assisted)
$57$86$171
Live-Cell Confocal
(Daily) 3
$300$380$795
Total Internal Reflection (TIRF)
(User)
$40$31$62$125
STORM Super-Resolution
(User)
$40$31$62$125
STORM Super-Resolution
(Daily)
$310$465$930
SIM Super-Resolution
(User)
$40$31$62$125
SIM Super-Resolution
(Daily)
$310$465$930
TIRF, STORM, SIM
(Assisted)
$58$90$181
Training (2 hour minimum)$80$120$235
     
ELECTRON MICROSCOPY    
TEM (user)$49$39$75$150
TEM (assisted)$75$115$230
FE-SEM (user)$40$31$62$125
FE-SEM (assisted)$60$88$175
FE-SEM (per day) 4$310$400$840
Dualbeam FIB-SEM (assisted)$75$112$225
Dualbeam FIB-SEM (per day) 4$750$1,125$2,250
CRYO-ELECTRON MICROSCOPY
Krios Cryo-EM (per hour)$110$165$330
Krios Cryo-EM (per day-trained) 5$1,760
Krios Cryo-EM (per day-assisted) 5$1,980$2,970$5,940
Glacios Cryo-EM (per hour)$90$135$270
Glacios Cryo-EM (per day-trained) 5$1,440
Glacios Cryo-EM (per day-assisted) 5$1,620$2,430$4,860
Aquilos 2 Cryo-FIB$75$113$225
Aquilos 2 Cryo-FIB (per day) 4$750$1,125$1,944
Training (3 hour minimum)$80$120$235
     
X-RAY MICROSCOPY    
Xradia Versa 520$70$55$105$210
Xradia Versa 520 (per day) 6$550$825$1,650
     
SAMPLE PREPARATION SERVICES    
Routine TEM
TEM Standard – Epoxy (1-4 samples)$200$300$600
– 5-9 samples$155$233$465
– 10+ samples$85$128$255
TEM Standard – Acrylic (1 -4 samples)$155$233$465
– 5-9 samples$130$195$390
– 10+ samples$70$105$210
TEM Immuno pre-embed (per sample)$245$368$735
TEM Tokyasu Immuno-label$297$446$891
Routine SEM / FIB-SEM
SEM Standard (per sample)$52$78$156
SEM Immune (per sample)$80$120$240
SEM Cassette (4 sample max)$155$233$465
FIB-SEM Standard (per sample)$280$420$840
Quick Freeze-Deep Etch
Liquid Helium Slam Freezing (per sample)$155$233$465
Deep Etch / Freeze Fracture (per replica)$187$281$561
Deep Etch / Freeze Fracture Immuno-label$255$383$765
   – Re-do fee (per replica)$105$158$315
Vitrification
High-Pressure Freezing (9 shots – includes 9 sapphire discs)$232$348$696
Freeze Substitution -Epoxy$83$125$249
Freeze Substitution – Acrylic$105$158$315
Plunge Freezing (per hour- trained)$20 —$ 30 $60
Plunge Freezing (per session – assisted)$40$60$120
Micro-ED sample prep (assisted – per grid)$8$15$30
Routine XRM
XRM Standard (per sample)$37$56$111
Tissue Clearing
iDISCO (per sample)$120$186$372
CLARITY (per sample)$170$255$510
Additional Services    
General Labor – per hour 7$70$109$217
Vibratome (per hour)$72$112$223
Thick-sectioning (routine) – per sample$86$133$267
Thin-sectioning (routine) – per hour$93$144$288
Cryo-sectioning (non-routine) – per hour$125$190$388
Negative Staining (per grid)$32$48$99
Negative Staining with immuno-labeling (per grid)$68$102$211
Negative Staining / Immuno-labeling/ Combination (EM Grid)$100$150$310
Immuno-labeling post sectioning TEM (per grid)$15$23$47
Critical Point Drying (per run)$37$56$115
Carbon Coating (per run)$37$56$115
Sputter Coating (per run)$37$56$115
Glow discharge (per run)$26$39$81
Reagents / Consumables
EM-grade Fixative / Buffer (up to 4 vials)$155$225$450
Sapphire discs (per disc)$42$60$120
Cryo-EM Grid$8$12$24
Cryo-EM Autogrids (per grid)$18$27$54
 Cryo-FIB Autogrids (per grid) $28 —$42  $84
DATA ANALYSIS    
Computer Workstation (per hour)$25$19$38$75
Computer Workstation (per day)$175$263$525
1Commercial access (during peak business hours only) to WUCCI resources will only be granted after research use agreement is in place.
2Peak time is defined as Monday – Friday, 8am – 6pm, off-peak is at all other times and over the weekend.
3Live-cell and Lightsheet imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half-day.
4FIB-SEM imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half day.
5Cryo-EM imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half-day.
6XRM imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half day.
7There will be a minimum one-hour general labor charge for all EM preps to cover consultation.