The Washington University Center for Cellular Imaging is engaged an active education program to support the effective training and subsequent development of its users advanced imaging and analysis skills. This comprises of a diversified portfolio of the following elements:

  • One-on-one and group training sessions on specific instrumentation and / or imaging modalities
  • Short courses comprised of both theoretical and practical elements
  • Lunch and Learn Seminars to introduce new technologies
  • Yearly Imaging Symposium in conjunction with other WUSTL stakeholders

User Training

All users will receive formal training by WUCCI staff before being allowed to use any center instruments. The WUCCI training policy operates as follows:

  1. An initial meeting is held with the user to discuss experimental needs, determine the most appropriate imaging system for their project, to provide a short orientation to the WUCCI, its policies and procedures and to assist the user with creating a WUCCI user account.
  2. A training session is scheduled between the user and a WUCCI staff member. This training session typically lasts ~3 hours and provides the user with an orientation on the basic operation of the microscope in question.At the end of this training session, the user is granted Level I access where they are able to book time on the microscope during business hours (Monday – Friday, 9am – 5pm) when a staff member is present to assist if needed.
  3. After 10 sessions, when the user has demonstrated competency in the safe operation of the microscope, they are granted Level II access when they can use the microscope 24/7 unsupervised.
  4. If additional training is required, i.e. for other advanced imaging modalities, they are scheduled with the scientific director, Dr. Fitzpatrick.

WUCCI Imaging Workshops

  • Live Cell and Super-Resolution Imaging (2-Day workshop) held in December 2015 and again in November 2016. The workshop was comprised of morning lectures on the theory and applications on the modalities and afternoon hands-on-training sessions on each instrument (in this case the Nikon A1R, Nikon Spinning Disk, Nikon SIM and Nikon TIRF/STORM imaging systems). Sponsored by Nikon Instruments.
  • Imaris 3D/4D Imaging Analysis (3-Day workshop) held in February 2016. The class covered topics such as 3D visualization approaches, object detection and tracking, cell lineage tracing, 2D and 3D co-localization analyses and neuron and vasculature tracing. The class consisted of morning lectures on the theory of each technique and afternoon hands-on-training sessions using the Bitplane Imaris software. Sponsored by Bitplane Software.
  • Correlative Light and Electron Microscopy (2-Day workshop) held in April 2016. This workshop covered correlative light and electron microscopy and single-molecular approaches such as Fluorescence Correlation Spectroscopy. The workshop was comprised of morning lectures on the theory and applications of the modalities and afternoon hands-on-training sessions on each instrument (in this case the Zeiss ApoTome and LSM 880 Airyscan imaging systems). Sponsored by Carl Zeiss Microscopy.
  • NIS Elements Image Processing Workshop  (2-Day workshop) to be held during February 7-9th 2017. This class will cover topics such as 3D visualization, deconvolution, co-localization analysis and object tracking using the NIS Elements software. The class will consist of morning theory classes and afternoon hand-on data analysis sessions. The workshop is sponsored by Nikon Instruments. Please contact James Fitzpatrick if you would like to register for this class.

Imaging Symposium

The WUCCI will be hosting a full-day Symposium on Cryo-Electron Microscopy (sponsored by FEI / Thermo Fisher) on May 2nd, 2017 at the Eric P. Newman Education Center at Washington University School of Medicine.