James A.J. Fitzpatrick, Ph.D.

Scientific Director, WUCCI

Professor, Departments of Neuroscience, Cell Biology & Physiology and Biomedical Engineering

Email: fitzp@wustl.edu, Tel: +1 (314) 747-0838

Academic CV

Biography

James Fitzpatrick, Ph.D., joined the School of Medicine on June 1st 2015 as the inaugural Scientific Director of the Washington University Center for Cellular Imaging (WUCCI) and as an Associate Professor of Neuroscience and Cell Biology & Physiology. He was promoted to Full Professor in 2019 and was awarded a Chan Zuckerberg Initiative Imaging Scientist award in 2020. Prior to his relocation to Washington University, he was Senior Director of Biophotonics and Strategic Technology Initiatives at the Salk Institute for Biological Studies in La Jolla, CA.

Dr. Fitzpatrick completed his undergraduate studies in Chemistry at Kings College, London and undertook graduate training in optical physics at the University of Bristol also in the United Kingdom. During his Ph.D. he designed and developed a novel injection seeded optical parametric oscillator (OPO) laser system for the study of nuclear hyperfine structure in the excited electronic states of gas phase free radical species. After completing his doctorate at the University of Bristol, he moved to the United States as a post-doctoral fellow at the University of Pittsburgh in Pennsylvania. There he shifted his focus to study biological molecules such as peptide mimics and their micro-solvated clusters in the gas-phase using high-resolution fluorescence spectroscopy. In his second post-doc at Carnegie Mellon University, also in Pittsburgh, he spent his time studying protein-protein interactions using tools such as fluorescence microscopy and fluorescence correlation spectroscopy (FCS). After completing his post-doctoral training, he joined the Carnegie Mellon National Technology Center for Networks and Pathways, an NIH funded Roadmap initiative whose mandate was to develop fluorescent probe and imaging informatics technologies to study networks and pathways in living cells.

Dr. Fitzpatrick’s primary research interests lie in the integration and application of multi-scale optical and charged particle imaging technologies — specifically, biological applications of ion microscopy, development of correlative 3D light and electron microscopy approaches, and new computational tools to visualize and manipulate large-scale multi-dimensional datasets.

Education
  • B.Sc. in Chemistry, King’s College London, U.K.
  • Ph.D. in Optical Physics, Laser Spectroscopy and Dynamics Group, University of Bristol, U.K.
  • NSF Postdoctoral Fellow, University of Pittsburgh, PA, USA
  • NIH Postdoctoral Fellow, Carnegie Mellon University, PA USA
Awards, Honors & Academic Memberships

2020 – present, Chan Zuckerberg Initiative Imaging Scientist

2020 – 2021, Vice-Chair, WUSM Executive Committee of the Faculty Council (DCFC)

2020 – present, Associate Editor, FASEB Bioadvances

2020 – present, Biological Sciences Director, Microscopy Society of America

2019 – 2020, Vice-Chair elect, WUSM Executive Committee of the Faculty Council 

2018 – present, Member, WUSM Executive Committee of the Faculty Council (ECFC)

2017 – present, Biological Symposium Organizer, Microscopy & Microanalysis Meeting

2013 Editor, ChemPhysChem Special Issue – Super-resolution Imaging and Nanophotonics ()

2011 – present, Associate Review Editor, Frontiers in Genomic Assay Technology

2009 Long-term quantum dot research featured in Chemical & Engineering News

2008 Cholera-toxin-B quantum dot research featured in Nature Nanotechnology

2008 FAP imaging technology featured in Nature Biotechnology

2002 Institute of Physics Research Travel Award

2001 and 2002 ICI “Excellence in Graduate Research” prize University of Bristol

Selected Publications
  • P.O. Bayguinov, M.R. Fisher, J.A.J. Fitzpatrick. 2020. Assaying Three-Dimensional Cellular Architecture using X-Ray Tomographic and Correlated Imaging Approaches. Journal of Biological Chemistry. 295(46):15782-15793.
  • Z. Deng, G. Maksaev, A.M. Schlegel, J. Zhang, M.J. Rau, J.A.J. Fitzpatrick, E.S. Haswell, P. Yuan. 2020. Structural mechanism for gating of a eukaryotic mechanosensitive channel of small conductance. Nature Communications.11(1):3690.
  • Z. Deng, G. Maksaev, M.J. Rau, Z. Xie, H. Hu, J.A.J. Fitzpatrick, P. Yuan. 2020. Gating of Human TRPv3 in a lipid bilayer. Nature Structure and Molecular Biology.27(7):635-644.
  • S. Pavlovic Djuranovic, J. Erath, R.J. Andrews, P.O. Bayguinov, J.J. Chung, J.A.J. Fitzpatrick, W.N. Moss, P. Szczesny, S. Djuranovic. 2020. Plasmodium falciparum translational machinery condones polyadenosine repeats. eLife 9:e57799.
  •  Z. Deng, Z. He, R.M. Bitter, M.J. Rau, J.A.J. Fitzpatrick, P. Yuan. 2020. Cryo-EM structures of the ATP release channel pannexin 1. Nature Structure and Molecular Biology. 27(4):373-381.
  • R. Hervas, M.J. Rau, Y. Park, W. Zhang, A.G. Murzin, J.A.J. Fitzpatrick, S.H.W. Scheres, K. Si. 2020. Cryo-EM structure of a neuronal functional amyloid implicated in memory persistence in Drosophila. Science. 13;367(6483):1230-1234.
  •  J.L. Krauss, P.M. Roper, A. Ballard, C.C. Shih, J.A.J. Fitzpatrick, J.E. Cassat, P.Y. Ng, N.J. Pavlos, D.J. Veis. 2019. Staphylococcus aureus Infects Osteoclasts and Replicates Intracellularly. MBio. Oct 15;10(5). pii: e02447-19.
  •  Y. Guang, T.M. McGrath, N. Klug, R. Nims, C.C. Shih, P.O. Bayguinov, F. Guilak, C.T.N. Pham, J.A.J. Fitzpatrick, L.A. Setton. 2019. Combined Experimental Approach and Finite Element Modeling of Small Molecule Transport Through Joint Synovium to Measure Effective Diffusivity. J Biomech Eng. Sep 1. doi: 10.1115/1.4044892
  •  Z. Yang, H. Yan, W. Dai, J. Jing, Y. Yang, S. Mahajan, Y. Zhou, W. Li, C. Macaubas, E.D. Mellins, C.C. Shih, J.A.J. Fitzpatrick, R. Faccio. 2019. Tmem178 negatively regulates store-operated calcium entry in myeloid cells via association with STIM1. Journal of Autoimmunity. S0896-8411(19)30042-3
  • K.X. Kim, S. Payne, A. Yang-Hood, S.Z. Li, B. Davis, J. Carlquist, B. V-Ghaffari, J.A. Gantz, D. Kallogjeri, J.A.J. Fitzpatrick, K.K. Ohlemiller, K. Hirose, M.A. Rutherford. 2019. Vesicular Glutamatergic Transmission in Noise-induced Loss and Repair of Cochlear Ribbon Synapses. J Neurosci. Mar 29: 2228-18
  •  H. Robles, S. Park, M.S. Joens, J.A.J. Fitzpatrick, C.S. Craft, E.L. Scheller. 2019. Characterization of the bone marrow adipocyte niche with three-dimensional electron microscopy. Bone. 118:89-98.
  • S.C. Rogers, L.B. Dosier, T.J. McMahon, H. Zhu, D. Timm, H. Zhang, J. Herbert, J. Atallah, G.M. Palmer, A. Cook, M. Ernst, J. Prakash, M. Terng, P. Towfighi, R. Doctor, A. Said, M.S. Joens, J.A.J. Fitzpatrick, G. Hanna, X. Lin, J.A. Reisz, T. Nemkov, A. D’Alessandro, A. Doctor. 2018. Red blood cell phenotype fidelity following glycerol cryopreservation optimized for research purposes. PLoS One. Dec 21;13(12):e0209201
  •  A. Cheruiyot, S. Li, A. Nickless, R. Roth, J.A.J Fitzpatrick, Z. You .2018. Compound C inhibits nonsense-mediated RNA decay independently of AMPK. PLoS One. Oct 5;13(10):e0204978.
  • A.M. Strubberg, D.A. Veronese Paniagua, T. Zhao, L. Dublin, T. Pritchard, P.O. Bayguinov, J.A.J. Fitzpatrick, B.B. Madison. 2018. The Zinc Finger Transcription Factor PLAGL2 Enhances Stem Cell Fate and Activates Expression of ASCL2 in Intestinal Epithelial Cells. Stem Cell Reports. Jul 9. pii: S2213- 6711(18)30272-8.
  • P.O. Baygionov, D.M. Oakley, C.C. Shih, D.J. Geanon, M.S. Joens and J.A.J. Fitzpatrick. 2018. Modern Laser Scanning Confocal Microscopy. Curr Protoc Cytom. Jul;85(1):e39. doi: 10.1002/cpcy.39.
  • M. Hoshi, A. Reginensi, M.S. Joens, J.A.J. Fitzpatrick, H. McNeill, S. Jain. 2018. Reciprocal Spatiotemporally Controlled Apoptosis Regulates Wolffian Duct Cloaca Fusion. J Am Soc Nephrol. Jan 11. pii: ASN.2017040380
  • C.C. Shih, D.M. Oakley, M.S. Joens, R.A. Roth and J.A.J. Fitzpatrick. 2018. Chapter 3: Non-Linear Optical Imaging of Extracellular Matrix Proteins. Methods in Cell Biology. 143:57-78.