WUCCI Training Request (Couch)

Name *

Email *

Phone Number

Position

PI Name *

Department

Training Type *

Widefield Fluorescence

Automated Slide Scanning (whole tissue)

Discrete Regions of Interest Imaging

Large Samples/ Macroscope

Brightfield (H&E, Nissl, CV, etc)

Polarized Light Microscopy

Unsure

Confocal Imaging Modalities

Multichannel Fluorescent Imaging

Colocalization of Specific Targets

Live Cell/Tissues

Thick Samples

FRET/FRAP

Fluorescence Correlation Spectroscopy

Reflectance

Unsure

Live Cell Confocal

Fast Imaging of Intracellular Processes

Imaging in Multiwell Plates or Plastic-Bottomed Dishes

Overnight Experiments

Unsure

Two-Photon Modalities

Super-resolution Imaging

Dissociated Cells, Standard Fluorophores

Tissue Sections

Stochastic Optical Reconstruction (requires photoswitchable fluorophores)

Unsure

Lightsheet Microscopy

Imaging of cleared tissues/ organoids

Fast Imaging of Intracellular Processes in Living Cells

Multidimensional analysis of developing organisms

Structural analysis of small organisms (Drosophila, C. elegance, etc.)

Electron Microscopy Type *

Microscopy Experience *

Yes

Microscopy Experience Description

Fluorescent Dye/Label

Sample Type *

Live

Fixed

Please describe what type of sample you'll be working with

Experiment Description *

Please describe your experimental paradigm. For light microscopy please describe whether you wish to image live-cells, do a z-stack or a FRAP or FRET experiment etc. For electron microscopy please describe what your desired outcome is (i.e. visualization of cellular ultrastructure, localization of a protein of interest etc).

Biosafety Compliance *

Yes

PLEASE NOTE: use of the WUCCI and its resources for the imaging of living BSl-2 samples or vertebrate animals WILL require an approved IBC/IACUC Protocol Addendum letter citing the WUCCI imaging site location. PLEASE provide BOTH the approved protocol number AS WELL AS upload a copy of the relevant IBC/IACUC protocol addendum as a part of this request.

File Upload *

Maximum file size: 51.2MB

Please upload study protocol

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