The WUCCI provides a complete service pipeline for light, electron, ion and 3D X-ray microscopy which is comprised of the following elements:

  • Affordable access to state-of-the-art light, electron, ion and 3D X-ray microscopy
  • Expert support in assay design, training on instrumentation and assistance with data acquisition
  • Analysis of multi-dimensional imaging datasets using commercial or custom-written algorithms

Imaging types available in the WUCCI

Light Microscopy

  1. Widefield Microscopy:   Multi-color fluorescence and DIC imaging of fixed samples.
  2. Confocal Microscopy:  Three-dimensional multi-color fluorescence imaging of fixed mounted cell cultures or tissue sections as well as live cell cultures and living specimens such as C. elegans and zebrafish model systems.
  3. Super-Resolution Microscopy: 2D / 3D localization microscopy, or Structured Illumination Microscopy of fixed cell cultures or thin tissue sections. 
  4. Two-Photon Microscopy: Three-dimensional multi-color fluorescence imaging of fixed mounted thick tissue sections as well as live specimens such as in vivo mouse imaging.

Electron / Ion Microscopy

  1. Transmission EM:  Ultrastructural nanoscale imaging of cell cultures and tissue samples as well as purified proteins / complexes.
  2. Scanning EM: Ultrastructural topographic imaging of cell cultures and tissue samples.
    • Focussed Ion Beam-SEM: Three-dimensional serial-block face (SBF) nanotomography of cell cultures and tissue samples.

    X-Ray Microscopy

    1. Non-invasive sub-micron-level resolution tomographic imaging of hard and soft materials from microns to many centimeters in size. 

    Cryo-EM

    • Single Particle Cryo-EM:  Atomic-level resolution reconstruction of macromolecular structures.
    • Cryo-Electron Tomography: Three-dimensional reconstruction of viral particles and thinned lamella of vitrified cell cultures.

    Requests

    Light Microscopy Training

    Electron Microscopy Training

    Cryo-EM Service Request

    SEM Sample Preparation

    TEM Sample Preparation

    XRM Sample Preparation

    FIB-SEM Sample Preparation

    Deep Etch EM Sample Preparation

    Other Services