WUCCI Rate Schedule – July 1st 2021 – June 30th 2022
| Washington University | Washington University | External Academic | Commercial 1 | |
| Peak 2 | Off-peak | Single-rate | Single-rate | |
| Light Microscopy | ||||
| Fluorescence / Slide Scanning | $28 | $21 | $42 | $84 |
| Confocal LSM | $39 | $30 | $59 | $118 |
| Two-Photon LSM | $39 | $30 | $59 | $118 |
| Lightsheet (inc. Lattice) (per hour) | $33 | $25 | $50 | $100 |
| Lightsheet (inc. Lattice) (per day) 3 | $200 | — | $300 | $600 |
| Live-Cell Confocal (per hour) | $37 | $28 | $56 | $112 |
| Live-Cell Confocal (per day) 3 | $225 | — | $338 | $676 |
| Total Internal Reflection (TIRF) | $39 | $30 | $59 | $118 |
| STORM Super-Resolution | $39 | $30 | $59 | $118 |
| SIM Super-Resolution | $39 | $30 | $59 | $118 |
| Training (2 hour minimum) | $75 | — | $115 | $225 |
| Electron Microscopy | ||||
| TEM (user) | $47 | $38 | $71 | $142 |
| TEM (assisted) | $71 | — | $107 | $214 |
| FE-SEM (user) | $39 | $30 | $59 | $118 |
| FE-SEM (assisted) | $55 | — | $82 | $164 |
| FE-SEM (per day) 4 | $240 | $360 | $720 | |
| Dualbeam FIB-SEM (assisted) | $71 | — | $107 | $214 |
| Dualbeam FIB-SEM (per day) 4 | $648 | — | $972 | $1,944 |
| Cryo-Electron Microscopy | ||||
| Krios Cryo-EM (per hour) | $110 | — | $165 | $330 |
| Krios Cryo-EM (per day-trained) 5 | $1,760 | — | — | — |
| Krios Cryo-EM (per day-assisted) 5 | $1,980 | — | $2,970 | $5,940 |
| Glacios Cryo-EM (per hour) | $90 | — | $135 | $270 |
| Glacios Cryo-EM (per day-trained) 5 | $1,440 | — | — | — |
| Glacios Cryo-EM (per day-assisted) 5 | $1,620 | — | $2,430 | $4,860 |
| Aquilos 2 Cryo-FIB | $71 | — | $107 | $214 |
| Aquilos 2 Cryo-FIB (per day) 4 | $648 | — | $972 | $1,944 |
| Training (3 hour minimum) | $75 | — | $115 | $225 |
| X-Ray Microscopy | ||||
| Xradia Versa 520 | $66 | $50 | $99 | $198 |
| Xradia Versa 520 (per day) 6 | $500 | — | $750 | $1,500 |
| Sample Preparation Services | ||||
| Routine TEM | ||||
| TEM Standard – Epoxy (1-4 samples) | $197 | — | $296 | $592 |
| – 5-9 samples | $150 | — | $225 | $450 |
| – 10+ samples | $80 | $120 | $240 | |
| TEM Standard – Acrylic (1 -4 samples) | $150 | — | $225 | $$50 |
| – 5-9 samples | $125 | — | $188 | $376 |
| – 10+ samples | $65 | — | $98 | $196 |
| TEM Immuno pre-embed (per sample) | $234 | — | $351 | $702 |
| Post-section Immuno-label on grid (up to 4 antibodies) | $65 | — | $98 | $196 |
| TEM Tokyasu Immuno-label | $284 | — | $426 | $852 |
| Routine SEM / FIB-SEM | ||||
| SEM Standard (per sample) | $50 | — | $75 | $150 |
| SEM Cassette (4 sample max) | $150 | — | $225 | $450 |
| FIB-SEM Standard (per sample) | $268 | — | $402 | $804 |
| Quick Freeze-Deep Etch | ||||
| Liquid Helium Slam Freezing (per sample) | $150 | — | $225 | $450 |
| Deep Etch / Freeze Fracture (per replica) | $180 | — | $270 | $540 |
| Deep Etch / Freeze Fracture Immuno-label | $245 | — | $368 | $736 |
| – Re-do fee (per replica) | $100 | — | $150 | $300 |
| Vitrification | ||||
| High-Pressure Freezing (9 shots – includes 9 sapphire discs) | $225 | — | $338 | $676 |
| Freeze Substitution -Epoxy | $80 | — | $120 | $240 |
| Freeze Substitution – Acrylic | $100 | $150 | $300 | |
| Plunge Freezing (per hour- trained) | $40 | — | $ 60 | $120 |
| Plunge Freezing (per grid – assisted) | $10 | — | $15 | $30 |
| Micro-ED sample prep (assisted – per grid) | $8 | — | $12 | $24 |
| Routine XRM | ||||
| XRM Standard (per sample) | $35 | — | $53 | $106 |
| XRM Immuno/EnzMet (per sample) | $100 | $150 | $300 | |
| Tissue Clearing | ||||
| iDISCO (per sample) | $115 | — | $233 | $466 |
| CLARITY (per sample) | $165 | — | $248 | $498 |
| Additional Services | ||||
| General Labor / Consultation – per hour 7 | $68 | — | $105 | $210 |
| Vibratome (per hour) | $70 | — | $105 | $210 |
| Thick-sectioning (routine) – per sample | $83 | — | $125 | $250 |
| Thin-sectioning (routine) – per hour | $90 | — | $135 | $270 |
| Cryo-sectioning (non-routine) – per hour | $120 | — | $180 | $360 |
| Negative Staining (per grid) | $30 | — | $45 | $90 |
| Immuno-labeling (per grid) | $65 | — | $98 | $196 |
| Negative Staining / Immuno-labelingl Combination (EM Grid) | $95 | — | $143 | $286 |
| Critical Point Drying (per run) | $35 | — | $53 | $106 |
| Carbon Coating (per run) | $35 | — | $53 | $106 |
| Sputter Coating (per run) | $35 | — | $53 | $106 |
| Glow discharge (per run) | $25 | — | $38 | $76 |
| Reagents / Consumables | ||||
| EM-grade Fixative / Buffer (up to 4 vials) | $150 | — | $225 | $450 |
| Sapphire discs (per disc) | $40 | — | $60 | $120 |
| Cryo-EM Autogrids (per grid) | $18 | — | $27 | $54 |
| Cryo-FIB Autogrids (per grid) | $28 | — | $42 | $84 |
| Data Analysis | ||||
| Computer Workstation (per hour) | $23 | $18 | $35 | $70 |
| Computer Workstation (per day) | $144 | — | $216 | $432 |
| 1 | Commercial access (during peak business hours only) to WUCCI resources will only be granted after research use agreement is in place. |
| 2 | Peak time is defined as Monday – Friday, 8am – 6pm, off-peak is at all other times and over the weekend. |
| 3 | Live-cell and Lightsheet imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half-day. |
| 4 | FIB-SEM imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half day. |
| 5 | Cryo-EM imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half-day. |
| 6 | XRM imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half day. |
| 7 | There will be a minimum one-hour general labor charge for all EM preps to cover consultation. |