WUCCI Rate Schedule – July 1st 2021 – June 30th 2022

 Washington UniversityWashington UniversityExternal AcademicCommercial 1
 Peak 2Off-peakSingle-rateSingle-rate
Light Microscopy    
Fluorescence / Slide Scanning$28$21$42$84
Confocal LSM$39$30$59$118
Two-Photon LSM$39$30$59$118
Lightsheet (inc. Lattice) (per hour)$33$25$50$100
Lightsheet (inc. Lattice) (per day) 3$200$300$600
Live-Cell Confocal (per hour)$37$28$56$112
Live-Cell Confocal (per day) 3$225$338$676
Total Internal Reflection (TIRF)$39$30$59$118
STORM Super-Resolution$39$30$59$118
SIM Super-Resolution$39$30$59$118
Training (2 hour minimum)$75$115$225
     
Electron Microscopy    
TEM (user)$47$38$71$142
TEM (assisted)$71$107$214
FE-SEM (user)$39$30$59$118
FE-SEM (assisted)$55$82$164
FE-SEM (per day) 4$240$360$720
Dualbeam FIB-SEM (assisted)$71$107$214
Dualbeam FIB-SEM (per day) 4$648$972$1,944
Cryo-Electron Microscopy
Krios Cryo-EM (per hour)$110$165$330
Krios Cryo-EM (per day-trained) 5$1,760
Krios Cryo-EM (per day-assisted) 5$1,980$2,970$5,940
Glacios Cryo-EM (per hour)$90$135$270
Glacios Cryo-EM (per day-trained) 5$1,440
Glacios Cryo-EM (per day-assisted) 5$1,620$2,430$4,860
Aquilos 2 Cryo-FIB$71$107$214
Aquilos 2 Cryo-FIB (per day) 4$648$972$1,944
Training (3 hour minimum)$75$115$225
     
X-Ray Microscopy    
Xradia Versa 520$66$50$99$198
Xradia Versa 520 (per day) 6$500$750$1,500
     
Sample Preparation Services    
Routine TEM
TEM Standard – Epoxy (1-4 samples)$197$296$592
– 5-9 samples$150$225$450
– 10+ samples$80$120$240
TEM Standard – Acrylic (1 -4 samples)$150$225$$50
– 5-9 samples$125$188$376
– 10+ samples$65$98$196
TEM Immuno pre-embed (per sample)$234$351$702
Post-section Immuno-label on grid (up to 4 antibodies)$65$98$196
TEM Tokyasu Immuno-label$284$426$852
Routine SEM / FIB-SEM
SEM Standard (per sample)$50$75$150
SEM Cassette (4 sample max)$150$225$450
FIB-SEM Standard (per sample)$268$402$804
Quick Freeze-Deep Etch
Liquid Helium Slam Freezing (per sample)$150$225$450
Deep Etch / Freeze Fracture (per replica)$180$270$540
Deep Etch / Freeze Fracture Immuno-label$245$368$736
   – Re-do fee (per replica)$100$150$300
Vitrification
High-Pressure Freezing (9 shots – includes 9 sapphire discs)$225$338$676
Freeze Substitution -Epoxy$80$120$240
Freeze Substitution – Acrylic$100$150$300
Plunge Freezing (per hour- trained)$40  —$ 60 $120
Plunge Freezing (per grid – assisted)$10$15$30
Micro-ED sample prep (assisted – per grid)$8$12$24
Routine XRM
XRM Standard (per sample)$35$53$106
XRM Immuno/EnzMet (per sample)$100$150$300
Tissue Clearing
iDISCO (per sample)$115$233$466
CLARITY (per sample)$165$248$498
Additional Services    
General Labor / Consultation – per hour 7$68$105$210
Vibratome (per hour)$70$105$210
Thick-sectioning (routine) – per sample$83$125$250
Thin-sectioning (routine) – per hour$90$135$270
Cryo-sectioning (non-routine) – per hour$120$180$360
Negative Staining (per grid)$30$45$90
Immuno-labeling (per grid)$65$98$196
Negative Staining / Immuno-labelingl Combination (EM Grid)$95$143$286
Critical Point Drying (per run)$35$53$106
Carbon Coating (per run)$35$53$106
Sputter Coating (per run)$35$53$106
Glow discharge (per run)$25$38$76
Reagents / Consumables
EM-grade Fixative / Buffer (up to 4 vials)$150$225$450
Sapphire discs (per disc)$40$60$120
Cryo-EM Autogrids (per grid)$18$27$54
 Cryo-FIB Autogrids (per grid) $28 —$42  $84
Data Analysis    
Computer Workstation (per hour)$23$18$35$70
Computer Workstation (per day)$144$216$432
1Commercial access (during peak business hours only) to WUCCI resources will only be granted after research use agreement is in place.
2Peak time is defined as Monday – Friday, 8am – 6pm, off-peak is at all other times and over the weekend.
3Live-cell and Lightsheet imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half-day.
4FIB-SEM imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half day.
5Cryo-EM imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half-day.
6XRM imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half day.
7There will be a minimum one-hour general labor charge for all EM preps to cover consultation.