WUCCI Rate Schedule as of March 1, 2023
| Washington University | Washington University | External Academic | Commercial 1 | |
| Peak 2 | Off-peak | Single-rate | Single-rate | |
| LIGHT MICROSCOPY | ||||
| Fluorescence / Slide Scanning (User) | $30 | $22 | $45 | $90 |
| Fluorescence / Slide Scanning (Assisted) | $44 | – | $65 | $131 |
| Fluorescence / Slide Scanning (Daily) | $220 | – | $330 | $660 |
| Confocal LSM (User) | $40 | $31 | $62 | $125 |
| Confocal LSM (Assisted) | $58 | – | $90 | $181 |
| Confocal LSM (Daily) | $310 | – | $465 | $930 |
| Two-Photon LSM (User) | $40 | $31 | $62 | $125 |
| Two-Photon LSM (Assisted) | $58 | – | $90 | $181 |
| Two-Photon LSM (Daily) | $310 | – | $465 | $930 |
| Lightsheet 7 (User) | $33 | $25 | $50 | $100 |
| Lightsheet 7 (Cleared samples- Assisted) | $36 | – | $54 | $108 |
| Lightsheet 7 (Aqueous samples- Assisted) | $48 | – | $72 | $144 |
| Lightsheet 7 (Daily)3 | $250 | – | $375 | $750 |
| Lattice Lightsheet (User) | $33 | $25 | $50 | $100 |
| Lattice Lightsheet (Assisted) | $36 | – | $54 | $108 |
| Lattice Lightsheet (Daily) | $250 | – | $375 | $750 |
| Live-Cell Confocal (User) | $39 | $30 | $60 | $120 |
| Live-Cell Confocal (Assisted) | $57 | – | $86 | $171 |
| Live-Cell Confocal (Daily) 3 | $300 | — | $380 | $795 |
| Total Internal Reflection (TIRF) (User) | $40 | $31 | $62 | $125 |
| STORM Super-Resolution (User) | $40 | $31 | $62 | $125 |
| STORM Super-Resolution (Daily) | $310 | – | $465 | $930 |
| SIM Super-Resolution (User) | $40 | $31 | $62 | $125 |
| SIM Super-Resolution (Daily) | $310 | – | $465 | $930 |
| TIRF, STORM, SIM (Assisted) | $58 | – | $90 | $181 |
| Training (2 hour minimum) | $80 | — | $120 | $235 |
| ELECTRON MICROSCOPY | ||||
| TEM (user) | $49 | $39 | $75 | $150 |
| TEM (assisted) | $75 | — | $115 | $230 |
| FE-SEM (user) | $40 | $31 | $62 | $125 |
| FE-SEM (assisted) | $60 | — | $88 | $175 |
| FE-SEM (per day) 4 | $310 | – | $400 | $840 |
| Dualbeam FIB-SEM (assisted) | $75 | — | $112 | $225 |
| Dualbeam FIB-SEM (per day) 4 | $750 | — | $1,125 | $2,250 |
| CRYO-ELECTRON MICROSCOPY | ||||
| Krios Cryo-EM (per hour) | $110 | — | $165 | $330 |
| Krios Cryo-EM (per day-trained) 5 | $1,760 | — | — | — |
| Krios Cryo-EM (per day-assisted) 5 | $1,980 | — | $2,970 | $5,940 |
| Glacios Cryo-EM (per hour) | $90 | — | $135 | $270 |
| Glacios Cryo-EM (per day-trained) 5 | $1,440 | — | — | — |
| Glacios Cryo-EM (per day-assisted) 5 | $1,620 | — | $2,430 | $4,860 |
| Aquilos 2 Cryo-FIB | $75 | — | $113 | $225 |
| Aquilos 2 Cryo-FIB (per day) 4 | $750 | — | $1,125 | $1,944 |
| Training (3 hour minimum) | $80 | — | $120 | $235 |
| X-RAY MICROSCOPY | ||||
| Xradia Versa 520 | $70 | $55 | $105 | $210 |
| Xradia Versa 520 (per day) 6 | $550 | — | $825 | $1,650 |
| SAMPLE PREPARATION SERVICES | ||||
| Routine TEM | ||||
| TEM Standard – Epoxy (1-4 samples) | $200 | — | $300 | $600 |
| – 5-9 samples | $155 | — | $233 | $465 |
| – 10+ samples | $85 | – | $128 | $255 |
| TEM Standard – Acrylic (1 -4 samples) | $155 | — | $233 | $465 |
| – 5-9 samples | $130 | — | $195 | $390 |
| – 10+ samples | $70 | — | $105 | $210 |
| TEM Immuno pre-embed (per sample) | $245 | — | $368 | $735 |
| TEM Tokyasu Immuno-label | $297 | — | $446 | $891 |
| Routine SEM / FIB-SEM | ||||
| SEM Standard (per sample) | $52 | — | $78 | $156 |
| SEM Immune (per sample) | $80 | – | $120 | $240 |
| SEM Cassette (4 sample max) | $155 | — | $233 | $465 |
| FIB-SEM Standard (per sample) | $280 | — | $420 | $840 |
| Quick Freeze-Deep Etch | ||||
| Liquid Helium Slam Freezing (per sample) | $155 | — | $233 | $465 |
| Deep Etch / Freeze Fracture (per replica) | $187 | — | $281 | $561 |
| Deep Etch / Freeze Fracture Immuno-label | $255 | — | $383 | $765 |
| – Re-do fee (per replica) | $105 | — | $158 | $315 |
| Vitrification | ||||
| High-Pressure Freezing (9 shots – includes 9 sapphire discs) | $232 | — | $348 | $696 |
| Freeze Substitution -Epoxy | $83 | — | $125 | $249 |
| Freeze Substitution – Acrylic | $105 | $158 | $315 | |
| Plunge Freezing (per hour- trained) | $20 | — | $ 30 | $60 |
| Plunge Freezing (per session – assisted) | $40 | — | $60 | $120 |
| Micro-ED sample prep (assisted – per grid) | $8 | — | $15 | $30 |
| Routine XRM | ||||
| XRM Standard (per sample) | $37 | — | $56 | $111 |
| Tissue Clearing | ||||
| iDISCO (per sample) | $120 | — | $186 | $372 |
| CLARITY (per sample) | $170 | — | $255 | $510 |
| Additional Services | ||||
| General Labor – per hour 7 | $70 | — | $109 | $217 |
| Vibratome (per hour) | $72 | — | $112 | $223 |
| Thick-sectioning (routine) – per sample | $86 | — | $133 | $267 |
| Thin-sectioning (routine) – per hour | $93 | — | $144 | $288 |
| Cryo-sectioning (non-routine) – per hour | $125 | — | $190 | $388 |
| Negative Staining (per grid) | $32 | — | $48 | $99 |
| Negative Staining with immuno-labeling (per grid) | $68 | — | $102 | $211 |
| Negative Staining / Immuno-labeling/ Combination (EM Grid) | $100 | — | $150 | $310 |
| Immuno-labeling post sectioning TEM (per grid) | $15 | $23 | $47 | |
| Critical Point Drying (per run) | $37 | — | $56 | $115 |
| Carbon Coating (per run) | $37 | — | $56 | $115 |
| Sputter Coating (per run) | $37 | — | $56 | $115 |
| Glow discharge (per run) | $26 | — | $39 | $81 |
| Reagents / Consumables | ||||
| EM-grade Fixative / Buffer (up to 4 vials) | $155 | — | $225 | $450 |
| Sapphire discs (per disc) | $42 | — | $60 | $120 |
| Cryo-EM Grid | $8 | $12 | $24 | |
| Cryo-EM Autogrids (per grid) | $18 | — | $27 | $54 |
| Cryo-FIB Autogrids (per grid) | $28 | — | $42 | $84 |
| DATA ANALYSIS | ||||
| Computer Workstation (per hour) | $25 | $19 | $38 | $75 |
| Computer Workstation (per day) | $175 | — | $263 | $525 |
| 1 | Commercial access (during peak business hours only) to WUCCI resources will only be granted after research use agreement is in place. |
| 2 | Peak time is defined as Monday – Friday, 8am – 6pm, off-peak is at all other times and over the weekend. |
| 3 | Live-cell and Lightsheet imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half-day. |
| 4 | FIB-SEM imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half day. |
| 5 | Cryo-EM imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half-day. |
| 6 | XRM imaging per day rate is for 24 hours of use. If imaging moves into a second 24-hour period, the charges for the second period will be charged to the half day. |
| 7 | There will be a minimum one-hour general labor charge for all EM preps to cover consultation. |